Coding

Part:BBa_K5048060:Design

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-23)


HSP60-FLAG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 325
    Illegal AgeI site found at 480
    Illegal AgeI site found at 958
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

HSP60 is a human protein expressed on the membrane of intestine epithelia. Codon optimization should be done to ensure the expression in E. coli, and a FLAG tag was added for easy detection. Cooperated with Neae and LAP, we expected to achieve cell-cell adhesion and cell-intestine adhesion in the small intestine(Fig 1).


Fig 1 The expected effect for the Adhesion module, which enables engineered bacteria to adhere to the intestine

Source

HSPD1 heat shock protein family D (Hsp60) member 1 [Homo sapiens (human)] - Gene - NCBI (nih.gov) CCD：CCDS Report for Consensus CDS (nih.gov)


References